Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Activation of Fn14 facilitates fibroblast senescence. A-D , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h. Cell samples were harvested after 48 h. Western blot for P53, P21, and γ-H2AX proteins expression ( n = 3). E , the expressions of Il6 , Tgfb1 , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA in primary fibroblasts were detected by Real-time PCR ( n = 3). F , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. G , Comet assay in rTWEAK-treated primary fibroblasts, scale bar = 10 μm. H , Immunofluorescence for Ki67 protein expression in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. I-J , Effects of Fn14 activation on the cell cycle were detected by flow cytometry. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Rabbit-anti-Ki67 polyclonal antibody , Proteintech , 27309-1-AP , 1:100.

Techniques: Activation Assay, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Staining, Single Cell Gel Electrophoresis, Immunofluorescence, Flow Cytometry

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Inhibition of the cGAS-STING signaling suppresses the senescence of rTWEAK-treated fibroblasts. A-B , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after RU.521 (10 µM) intervention for 30 min. Western blot was conducted to examine the expression levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 ( n = 3). C-D , The senescence-related protein levels of P53, P21, P16, and γ-H2AX were assessed by Western blot ( n = 3). E , Immunofluorescence of Ki67 protein expression in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 50 μm. F , Comet assay in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 10 μm. G , Real-time PCR for Il-6 , Tgf-β , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression ( n = 3). H , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Rabbit-anti-Ki67 polyclonal antibody , Proteintech , 27309-1-AP , 1:100.

Techniques: Inhibition, Western Blot, Expressing, Immunofluorescence, Single Cell Gel Electrophoresis, Real-time Polymerase Chain Reaction, Staining

Journal: bioRxiv

Article Title: Spatially precise neuron formation via hydrogel mediated modulation of the host astrocyte response

doi: 10.64898/2025.12.20.695501

Figure Lengend Snippet: A representative image of astrocytes transduced with A, F) AAV-mCherry and B, E, G) AAV-SOX2 and stained with nestin/OCT3-4/Ki67 marker (cyan), mCherry (red), GFAP (yellow), and DAPI (blue). C) Quantification data showing the population of nestin+, mCherry+, and double positive cells (both nestin+ and mCherry+) in culture. H) Quantification data showing the population of ki67 positive cells, proliferation marker, in AAV-mCherry and AAV-SOX2-mCherry transduced cells. I) The percentage of confluence for AAV-SOX2-mCherry and AAV-mCherry transduced cells at any given time point acquired using IncuCyte live imaging. J) Cell cycle analysis on AAV-SOX2-mCherry and AAV-mCherry transduced astrocytes showing significant increase in S phase and G2/M phase of SOX2 transduced cells indicating the presence proliferating cells. Data represents Mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 . N=3-5 independent cultures/group. Scale bar=100 µm.

Article Snippet: The following primary antibodies (neural and astrocyte markers) were used: mouse monoclonal anti-β3-tubulin/TUJ1, (1:500, biolegend), goat monoclonal anti-Glial Fibrillary Acidic Protein (GFAP, 1:500, Abcam), chicken polyclonal anti-mCherry (mCherry, 1:1000, Abcam), rabbit monoclonal anti-NeuN (Neun, 1:1000, Abcam)/ rabbit monoclonal anti-Microtubule-associated protein 2 (MAP2, 1:1000, Abcam), rabbit polyclonal anti-ki67 (1:300, Novus), and rabbit polyclonal anti-OCT3/4 (1:100, Invitrogen).

Techniques: Transduction, Staining, Marker, Imaging, Cell Cycle Assay

Journal: Journal of Translational Medicine

Article Title: Sodium-myoinositol cotransporter-1 downstream of m6A methyltransferase WTAP exerts a potential carcinogenicity in diffuse large B-cell lymphoma progression

doi: 10.1186/s12967-025-07303-7

Figure Lengend Snippet: SMIT1 accelerates DLBCL cell-derived tumor xenograft growth in vivo. ( A ) Growth curves of tumor xenografts formed by U2932 cells bearing oeSMIT1 and shSMIT1. ( B ) Photographs of tumor xenografts on day 24 after implantation. ( C ) Tumor weight of xenografts on day 24 after implantation. ( D ) Western blot assay showing the expression of SMIT1 in tumors formed by U2932 cells. ( E ) IHC staining showing Ki67 expression in tumors formed by U2932 cells. ( F ) TUNEL staining in tumors formed by U2932 cells. Data are presented as mean ± SD. † p < 0.05, †† p < 0.01, and ††† p < 0.001

Article Snippet: Subsequently, the sections were incubated with anti-Ki67 polyclonal rabbit antibody (1:200 dilution, Proteintech Group Inc., Rosemont, IL, USA, 27309-1-AP) or anti-phospho-AKT-S473 monoclonal rabbit antibody (1:50 dilution, ABclonal, AP1208) at 4°C overnight, followed by the incubation of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution, ThermoFisher SCIENTIFIC, Pittsburgh, PA, USA, 31460) for 1 h at 37°C.

Techniques: Derivative Assay, In Vivo, Western Blot, Expressing, Immunohistochemistry, TUNEL Assay, Staining